Anti-proliferative Drug Assays – AML
The Native Environment (NE) proliferation assays are performed for those compounds in which cell cycle arrest is suspected to be more relevant than depletion. The drug effect is measured on both the proliferating and nonproliferating cell subsets. For cell proliferation analysis, CFDA staining in conjunction with the immunophenotype allow the analysis of both viability (Annexin-V negative) and proliferation (CFDA peaks) of the blast cells.
Tubeandbracket
  • AML
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • AML blasts
  • Lymphocytes
  • Others

Cytotoxic Drug Therapy
  • Blast Proliferation Induction
Plate Cytotoxic Drug

Hematological
Sample

Native Cell
Population

Drug Incubation
FLT3L + SCF + IL3 + IL6

Cells Retrieval &
FCM Readout

Anti-proliferative Drug Assays – AML:
Native Environment

001 Anti Proliferative Decitabine Assay
Hematological
Sample
Tubeandbracket
  • AML
Native Cell Population
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • AML blasts
  • Lymphocytes
  • Others
Drug Incubation FLT3L + SCF + IL3 + IL6
Anti-Proliferative Drugs Therapy
Cytotoxic Drug Therapy
  • Blast Proliferation Induction
Cells Retrieval & FCM Readout
Plate Cytotoxic Drug
Anti-proliferative Drug Assays – AML:
Native Environment
001 Anti Proliferative Decitabine Assay