Bispecific Antibodies Testing
The Tumor Microenvironment (TME) has a key role in Solid Tumor Therapy Screening. The 3D TME-aligned model recapitulates original tumor microenvironment and promotes naïve behavior of tumor and immune cells. Once embedded in this native-like matrix, T-cells and tumor cells interact through immunological synapses potentialized by Bispecific (or multispecific) antibodies. The cell retrieval at the end of treatment allows full mode of action characterization by FCM and description of genetic basis of resistance.
- Tumor cells
- T cells / TILs
- TME-aligned 3D matrix & media
Bispecific Antibodies
Tumor Cells
hMSC factors
T-Cells / TILs
- Tumor cell lines
- Healthy T-cells
- TILs
- hMSC media
- Inflammatory TME
- Acidic pH / Low glucose
- Desired E:T ratios
- Reliable Drug Response
- Cells Retrieval
- Tumor cells numbers
- T-cell profiling
Cells Source
Naïve-like Tumor Microenvironment:
TME-aligned 3D Model
Cells Retrieval
& FCM Readout
Ex-vivo Bispecific Ab Screening:
TME-aligned 3D IO Model
- Up to 11 colour markers – full cell profiling
- Good statistics & reproducibility
- Low time consuming
Cells Source
- Tumor cell lines
- Patient Tumor Cells
- Healthy T-cells
- Autologous TILs
- hMSC media
- Tumor cells
- T cells / TILs
- TME-aligned 3D matrix & media
Naïve-like Tumor Microenvironment:
TME-aligned 3D Model
- Inflammatory TME
- Acidic pH / Low glucose
- Desired E:T ratios
- Reliable Drug Response
Bispecific Antibodies
Tumor Cells
hMSC factors
T-Cells / TILs
Cells Retrieval
& FCM Readout
& FCM Readout
- Cells Retrieval
- Ki67 / EdU
- Molecular bases of resistance
Ex-vivo Bispecific Ab Screening:
TME-aligned 3D IO Model
TME-aligned 3D IO Model
- Up to 7 colour markers – full cell profiling
- Good statistics & reproducibility
- Low time consuming
- FACS sorting functionally relevant cells
- Molecular on all or cell subsets
- MoA, signaling
