Bispecific Antibodies Testing
The Tumor Microenvironment (TME) has a key role in Solid Tumor Therapy Screening. The 3D TME-aligned model recapitulates original tumor microenvironment and promotes naïve behavior of tumor and immune cells. Once embedded in this native-like matrix, T-cells and tumor cells interact through immunological synapses potentialized by Bispecific (or multispecific) antibodies. The cell retrieval at the end of treatment allows full mode of action characterization by FCM and description of genetic basis of resistance.
Tumorcell
  • Tumor cells
T Cells Tils Solid Tumor
  • T cells / TILs
Tme Aligned 3d
Tme Tumor Media
  • TME-aligned 3D matrix & media

Antibody F2488C
Cytotoxic Compund Solid Tumor
Single Tumorcell

Tumor Cells

Single Hmscfactors

hMSC factors

T Cells TILs

T-Cells / TILs

Plate Tumor Solid
  • Tumor cell lines
  • Patient Tumor Cells
  • Healthy T-cells
  • Autologous TILs
  • hMSC media
  • Inflammatory TME
  • Acidic pH / Low glucose
  • Desired E:T ratios
  • Reliable Drug Response
  • Cells Retrieval
  • Tumor cells numbers
  • T-cell profiling

Cells Source

Naïve-like Tumor Microenvironment:
TME-aligned 3D Model

Cells Retrieval
& FCM Readout

Ex-vivo Bispecific Ab Screening:
TME-aligned 3D IO Model

Graf Bispecific
  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling
Cells Source
  • Tumor cell lines
  • Patient Tumor Cells
  • Healthy T-cells
  • Autologous TILs
  • hMSC media
Tumorcell
  • Tumor cells
T Cells Tils Solid Tumor
  • T cells / TILs
Tme Aligned 3d
Tme Tumor Media
  • TME-aligned 3D matrix & media
Naïve-like Tumor Microenvironment: TME-aligned 3D Model
  • Inflammatory TME
  • Acidic pH / Low glucose
  • Desired E:T ratios
  • Reliable Drug Response

Antibody F2488C
Cytotoxic Compund Solid Tumor
Single Tumorcell

Tumor Cells

Single Hmscfactors

hMSC factors

T Cells TILs

T-Cells / TILs

Cells Retrieval
& FCM Readout
  • Cells Retrieval
  • Ki67 / EdU
  • Molecular bases of resistance
Plate Tumor Solid
Ex-vivo Bispecific Ab Screening:
TME-aligned 3D IO Model
Graf Bispecific
  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling