Cytotoxic Drugs Assays: Native Environment
The Native Environment (NE) recapitulates naïve tumor behavior and promotes realistic drug responses. NE contains key factors such as tumor-stromal interactions, plasma, platelets, or red blood cells (RBC) that will influence the clinical efficacy of many drugs. The PharmaFlow platform allows us to aspirate the same exact volume and content of each well from a 96 well-plate to the flow of the cytometer. The combination of fluorescently labeled antibodies to identify leukemic cells and Annexin-V staining to exclude dying cells, allows us to measure differences in the number of live leukemic cells in a well with drugs vs. the control wells. In this way, the PharmaFlow platform can quantify the activity for all those drugs that produce programmed cell death or a reduction in the number of target cells.
Tubeandbracket
  • AML
  • CLL
  • MM
  • Others
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • Neutrophils
  • AML / CLL / MM Leukemic Cells
  • Lymphocytes
  • Others

Cytotoxic Drug Therapy
Plate Cytotoxic Drug

Hematological
Sample

Native Cell
Population

Drug
Incubation

Cells Retrieval &
FCM Readout

Cytotoxic Drugs Assays:
Native Environment

Average 8 AML drugs
Hematological
Sample
Tubeandbracket
  • AML
  • CLL
  • MM
  • Others
Native Cell Population
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • Neutrophils
  • AML / CLL / MM Leukemic Cells
  • Lymphocytes
  • Others
Drug Incubation
Cytotoxic
Drug
Therapy
Cytotoxic Drug Therapy
Cells Retrieval & FCM Readout
Plate Cytotoxic Drug
Cytotoxic Drugs Assays: Native Environment
Average 8 AML drugs