Cytotoxic Compounds Testing
The Tumor Microenvironment (TME) has a key role in Solid Tumor Therapy Screening. The 3D TME-aligned model recapitulates original tumor microenvironment and promotes naïve behavior of tumor cells. Once re-educated in this native-like matrix, the tumor cells respond in a superiorly faithful manner to cytotoxic compounds. The cell retrieval at the end of treatment allows full mode of action characterization by FCM and description of genetic basis of resistance.
Tumorcell
  • Tumor cells
Tme Aligned 3d
  • TME-aligned 3D matrix
Tme Tumor Media
  • TME-aligned tumor media

Cytotoxic Compund Solid Tumor
Single Tumorcell

Tumor Cells

Single Hmscfactors

T-Cells Activation

  • Inflammatory TME
  • Acidic pH / Low glucose
  • Reliable Drug Response
Plate Tumor Solid
  • Cells Retrieval
  • Ki67 / EdU
  • Molecular bases of resistance

Tumor Cell Lines
or Patient Samples

Naïve-like Tumor Microenvironment:
TME-aligned 3D Model

Cells Retrieval
& FCM Readout

Ex-vivo Drug Screening:
TME-aligned 3D Model

  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling
  • Epigenetic readouts
Tumor Cell Lines
or Patient Samples
Tumorcell
  • Tumor cells
Tme Aligned 3d
  • TME-aligned 3D matrix
Tme Tumor Media
  • TME-aligned tumor media
Naïve-like Tumor Microenvironment: TME-aligned 3D Model

Cytotoxic Compund Solid Tumor
Single Tumorcell

Tumor Cells

Single Hmscfactors

T-Cells Activation

  • Inflammatory TME
  • Acidic pH / Low glucose
  • Reliable Drug Response
Cells Retrieval
& FCM Readout
Plate Tumor Solid
  • Cells Retrieval
  • Ki67 / EdU
  • Molecular bases of resistance
Ex-vivo Drug Screening:
TME-aligned 3D Model
  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling
  • Epigenetic readouts