Compound Profiling by Flow Cytometry

 
Inmunophenotyping and Receptor expression​

  • Categorized analysis of population and subtypes identified by flow cytometry
  • Receptor quantification
  • Dot-plots and histograms from cytometry analysis
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Pharmacological Effects from Ex Vivo Assays

  • Analysis of pharmacological cytotoxic and antiproliferative effects of single drugs over cell populations and subpopulations identified by flow cytometry
  • Dose-response curve fitting and drug effect normalization
  • Pharmacodynamic parameters estimation. Area under the curve
  • Categorical analysis according to different variables: experimental conditions, subpopulations identified by different markers...
  • Comparative analysis among drugs tested under same experimental conditions
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  • Characterization of population pharmacological effects of drugs through Pharmacodynamic Mixed-Effects Non-linear population models using NONMEM® software. Estimation of population typical parameters and associated inter-patient variability together with model quality parameters and errors estimations.
Population VPC
  • Estimation of synergy in the pharmacological effects (cytotoxic or antiproliferative) observed in combination experiments using two drugs
  • Empirical estimation using Combination Index
  • Color maps for estimation of optimal synergistic dose-ratios
  • Modeling using Interaction Surface approach. 3D Surface plots
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