Compound Profiling by Flow Cytometry
Inmunophenotyping and Receptor Expression Pharmacological Effects and Synergism
from Ex Vivo Assays

 
Inmunophenotyping and Receptor expression​

  • Categorized analysis of population and subtypes identified by flow cytometry
  • Receptor quantification
  • Dot-plots and histograms from cytometry analysis
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Pharmacological Effects from Ex Vivo Assays

  • Analysis of pharmacological cytotoxic and antiproliferative effects of single drugs over cell populations and subpopulations identified by flow cytometry
  • Dose-response curve fitting and drug effect normalization
  • Pharmacodynamic parameters estimation. Area under the curve
  • Categorical analysis according to different variables: experimental conditions, subpopulations identified by different markers...
  • Comparative analysis among drugs tested under same experimental conditions
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  • Characterization of population pharmacological effects of drugs through Pharmacodynamic Mixed-Effects Non-linear population models using NONMEM® software. Estimation of population typical parameters and associated inter-patient variability together with model quality parameters and errors estimations.
Population VPC
  • Innovative Drug Interaction Studies. Drug combinations are critical in many diseases, including cancer. Synergistic drugs allow the use of lower doses in the treatment and therefore the adverse reactions would probably be reduced.
    Vivia has developed a workflow including different methods like traditional dose dependent indexes, surface modeling (Greco et al., 1995) and the new MuSyC2,3 algorithm to perform a complete drug interaction study.
  • Innovative workflow using a complete set of synergy calculation methods.
  • Pre-analysis phase to maximize per well information collection without quality loss. (Single drug & combination doses selection, incubation time…)
  • Efficacy and cooperativity synergy added to the traditional potency synergy.
  • Asymmetric synergy detection (α12, α21, γ12 & γ21)
  • Interactive Spotfire synergy data analysis and visualization (3D Surface viewer, drug interaction parameters, synergy score, single drug dose response curves …)
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