Migration and Invasion Assays
The Tumor Microenvironment (TME) has a key role in Solid Tumor Therapy Screening. TME-aligned model recapitulates original tumor microenvironment and promotes naïve behavior of tumor cells. Once embedded in this native-like matrix, tumor cells can migrate and invade, crucial steps for solid tumor metastasis in vivo. This assay enables evaluation of drugs that affects migratory capacity of tumor cells. The cell retrieval at the end of invasive process allows full mode of action characterization by FCM.

Therapy

Migration Therapy

Stimulus-directed
Migration

Migration Stimulus

Cell
Retrieval

Migration Cell Retrieval
Plate Tumor Solid
  • Tumor cell lines
  • Patient Tumor Cells
  • hMSC media
  • Inflammatory TME
  • Acidic pH / Low glucose
  • Reliable Drug Response
  • Cells Retrieval
  • Tumor cells numbers
  • Molecular Bases of Resistance

Cells Source

Naïve-like Tumor Microenvironment:
TME-aligned 3D Model

Cells Retrieval
& FCM Readout

Ex-vivo Bispecific Ab Screening:
TME-aligned 3D IO Model

  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling
  • Epigenetic readouts
Cells Source
  • Tumor cell lines
  • Patient Tumor Cells
  • hMSC media

Therapy

Migration Therapy
Naïve-like Tumor Microenvironment: TME-aligned 3D Model
  • Inflammatory TME
  • Acidic pH / Low glucose
  • Reliable Drug Response

Stimulus-directed
Migration

Migration Stimulus

Cell
Retrieval

Migration Cell Retrieval
Cells Retrieval
& FCM Readout
  • Cells Retrieval
  • Tumor cells numbers
  • Molecular Bases of Resistance
Plate Tumor Solid
Ex-vivo Migration & Invasion Assays:
TME-aligned Model
  • Up to 7 colour markers – full cell profiling
  • Good statistics & reproducibility
  • Low time consuming
  • FACS sorting functionally relevant cells
  • Molecular on all or cell subsets
  • MoA, signaling
  • Epigenetic readouts