Antibody-dependent Cellular cytotoxicity (ADCC)
The Antibody-Dependent Cellular Cytotoxicity (ADCC) Assay incorporates real Effector to Target ratios (NK Cells: Leukemic Cells) to capture the activity of these monoclonal antibodies mediated by cells displaying Fc receptors, such as NK cells and macrophages, evaluating their depletion results based on the quantification of live cells. Initial target quantification is evaluated by Flow Cytometry to correlate with the ex vivo activity.
Tubeandbracket
  • AML
  • CLL
  • MM
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • AML / CLL / MM Leukemic Cells
  • Natural Killer Cells
  • Others

Natural Killer Cells Activation

Cytotoxic Drug Therapy
Plate Cytotoxic Drug

Hematological
Sample

Native Cell
Population

Antibody Incubation
NK Cells Activation

Cells Retrieval &
FCM Readout

CDC Assay:
Native Environment

004 ADCC Daratumumab Assay 3h 6h & 24h
Hematological
Sample
Tubeandbracket
  • AML
  • CLL
  • MM
Native Cell Population
Native Blook Cell Populations
  • Red Blood Cells
  • Platelets
  • AML / CLL / MM Leukemic Cells
  • Natural Killer Cells
  • Others
Antibody Incubation NK Cells Activation
Antibodies
Therapy

Natural Killer Cells Activation

Cytotoxic Drug Therapy
Cells Retrieval & FCM Readout
Plate Cytotoxic Drug
CDC Assay:
Native Environment
004 ADCC Daratumumab Assay 3h 6h & 24h