Anti-proliferative Drug Assays – CLL
To more closely reproduce and re-create the complexity of the in vivo microenvironment in CLL, we have developed an ex vivo assay that best promotes CLL proliferation and viability with the combination of CpG + IL-2 + HS5 stromal cells, that approximates a lymph node environment. Human stromal cel lines have been reported to prevent spontaneous death and promote proliferation of tumor cells.

- CLL

- Red Blood Cells
- Platelets
- B-CLL Cells
- Lymphocytes
- Others
Anti-Proliferative Drugs Therapy
T-Cells
Activation

- B-Cell Proliferation Induction

Hematological
Sample
Native Cell
Population
Drug Incubation
CpG + IL2 + HS5 Cell Line
Cells Retrieval &
FCM Readout
Anti-proliferative Drug Assays – CLL:
Native Environment

Hematological
Sample
Sample

- CLL
Native Cell
Population

- Red Blood Cells
- Platelets
- B-CLL Cells
- Lymphocytes
- Others
Drug Incubation
CpG + IL2 + HS5 Cell Line
Anti-Proliferative Drugs Therapy
T-Cells Activation

- B-Cell Proliferation Induction
Cells Retrieval &
FCM Readout

Anti-proliferative Drug Assays – CLL:
Native Environment
Native Environment
