The controlled expansion of T cells is a critical component of immune activation in both protective and pathological contexts. Monitoring proliferation under defined stimulatory conditions allows for the evaluation of drug effects on T-cell dynamics, particularly in autoimmune and inflammatory diseases. This assay provides a functional readout of compound impact on immune activation and potential immunosuppression.

In autoimmune diseases, the balance between naïve and memory T cell subsets reflects immune activation, chronic stimulation, and dysregulation. Characterizing these populations is key to understanding disease mechanisms and assessing the immunological impact of therapeutic interventions.

Polyclonal stimulation provides a robust model to assess T-cell functionality and drug effects under standardized activation conditions. This assay is especially useful to explore T-cell behavior in autoimmune settings, including activation, exhaustion, cytokine release, and effector function.

A functional tool to assess immune activation and modulation in B- and T-cell–mediated autoimmune responses under native ex vivo conditions.